SD-39 Agar is used in the presumptive enumeration of Escherichia coli O157:H7, and used with ISO-GRID® and/or NEO-GRID® Membrane Filtration System. SD-39 Agar conforms to AOAC Method #997 for food commodities in a laboratory setting. SD-39 Agar is not intended for use in the diagnosis of disease or other conditions in humans.
SD-39 Agar was formulated for direct presumptive enumeration of Escherichia coli O157:H7, and its direct differentiation from most other E. coli. SD-39 Agar combines some features of Lactose Monensin Glucuronate Agar and EF-18 Agar. Formula modifications include the addition of a chromogenic substrate for detection of β-glucuronidase activity and substitution of sorbitol for lactose or sucrose as a fermentable carbohydrate. The formula also enables direct differential enumeration of β-glucuronidase-positive E. coli.
SD-39 Agar is recommended for the direct, presumptive enumeration of E. coli O157:H7 in meats, poultry, dairy foods, infant formula, liquid eggs, mayonnaise, and apple cider. This medium is also used for the direct enumeration of β-glucuronidase-positive E. coli from foods.
|Enzymatic Digest of Animal Tissue||5 g|
|Yeast Extract||3 g|
|Sodium Chloride||5 g|
|Magnesium Sulfate||1.5 g|
|Sodium Deoxycholate||0.15 g|
|Sodium Glucuronate||0.5 g|
|Phenol Red||0.12 g|
Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
- Suspend 63 g of the medium in one litre of purified water.
- Heat with frequent agitation to boiling to completely dissolve the medium.
- DO NOT AUTOCLAVE.
|Product #||Product Description|
|6907A||SD-39 Agar, 500 g|
Please contact Neogen’s regulatory department at SDS@neogen.com for SDS information.