SCCRAM Broth is a non-selective culture medium used for the rapid enrichment of Salmonella spp. with the ISO-GRID® and/or NEO-GRID® Membrane Filtration System in a laboratory setting. SCCRAM Broth is not intended for use in the diagnosis of disease or other conditions in humans.

SCCRAM Broth was developed for the primary non-selective enrichment of Salmonella spp. from food samples. This medium was designed to stimulate sufficient repair and multiplication of Salmonella spp. within 6 – 8 hours of incubation at 42°C. The SCCRAM Broth formulation is used to enable direct presumptive detection of Salmonella in a total incubation period of 24 hours using EF-18 Agar and the ISO-GRID and/or NEO-GRID Membrane Filtration System.

 

Formula Litre
Special Peptone 10 g
Sodium Pyruvate 5 g
Sodium Chloride 5 g
MOPS, free acid 10.5 g
MOPS, sodium salt 11.6 g

Final pH: 7.1 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplement, if desired

Tween® 80, 100 mL

Directions

  1. Suspend 42.1 g of the medium in one litre of purified water.
  2. If desired, add 10 g of Tween 80.
  3. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  4. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
6910A SCCRAM Broth, 500 g
7992 Tween 80, 100 mL

Reference Material

6910 - SCCRAM Broth, Product Information Sheet [English]
6910 - SCCRAM Broth, Safety Data Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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