The Reveal® 3-D Food Allergen tests are uniquely designed with 3 lines of detection and can be used virtually anywhere to screen environmental swabs and rinses for the presence of target allergens. The test’s 3-D technology ensures greater reliability with screening than ever before.
The Reveal 3-D format is a single-step lateral flow immunochromatographic assay. The extract is wicked through a reagent zone, which contains antibodies specific for the target allergen conjugated to coloured particles. If allergen is present, it will be captured by the conjugated antibodies. The allergen antibody-particle complex then is wicked onto a membrane which contains a zone of antibody specific for the target allergen. This zone captures the complex allowing the particles to concentrate and form a visible line. If no target allergen is present, no line will form.
Additionally, the 3-D format has a unique feature to guard against oversaturation. The second line, referred to as the overload line, will disappear in situations where gross contamination is observed preventing false negative results from occurring. The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of the target allergen, ensuring the strip is working properly.