LMG Agar (Lactose Monensin Glucuronate Agar) is used in the selective and differential isolation of coliform bacteria, using the ISO-GRID® and /or NEO-GRID® Membrane Filtration System. LMG Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Lactose Monensin Glucuronate Agar is based on the coliform enumeration medium, m-FC Agar. The original m-FC formula was designed for faecal coliform enumeration from water samples using membrane filtration. This medium was modified for enumeration of total coliforms by eliminating rosolic acid supplement and reducing the incubation temperature. A formula change to modified m-FC Agar included the addition of Monensin to improve recovery of injured coliforms.

LMG Agar is recommended for the detection and enumeration of total confirmed coliform bacteria from all foods using the ISO-GRID and /or NEO-GRID Membrane Filtration System method.

 

Formula Litre
Enzymatic Digest of Casein 10 g
Enzymatic Digest of Animal Tissue 5 g
Yeast Extract 3 g
Lactose 12.5 g
Monensin 0.038 g
Sodium Deoxycholate 0.15 g
Aniline Blue 0.1 g
Glucuronic Acid, Sodium Salt 0.5 g
Agar 15 g

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 46.2 g of the medium in one litre of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. DO NOT AUTOCLAVE.
  4. Cool to 45 – 50° C.
  5. Determine pH and adjust if necessary to 7.2 ± 0.2.

Order Details

Product # Product Description
6902A LMG Agar, 500 g

Reference Material

6902 - LMG Agar, Product Information Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

Please check with your local representative for availability of these products within your region.