Agri-Screen® diagnostics kits from Neogen® are screening test systems that can be interpreted visually. Samples are compared to a control or colour chart.
Agri-Screen for Mycotoxins
Each test kit contains antibody-coated microwells with antibodies specific to the kit’s target substance.
First, samples and controls are added to their respective test wells. Next, an enzyme conjugate (target substance chemically linked with an enzyme) is added. The samples/controls and conjugate are mixed and transferred to antibody wells where they compete for the antibody binding sites. The more target substance in the sample, the less conjugate that binds in the wells.
After incubation, the wells are washed to remove all unbound materials.
A substrate, which changes colour in the presence of the conjugate, is added to the wells. During incubation, blue colour develops in proportion to the amount of conjugate versus target substance in the wells. The more conjugate bound, the more blue colour that develops, indicating less substance present.
Results are read visually in a screening format—the less blue colour, or more red, the more target substance detected.