Product number: 7359
Violet Red Bile Agar with MUG is used for the detection of coliforms and the fluorogenic detection of Escherichia coli in a laboratory setting. Violet Red Bile Agar with MUG is not intended for use in the diagnosis of disease or other conditions in humans.
Violet Red Bile Agar with MUG is specified in many procedures to enumerate coliforms in food and dairy products. In 1982, Feng and Hartman developed a rapid fluorogenic assay for Escherichia coli by incorporating 4-methylumbelliferyl-β-D-glucuronide (MUG) into Lauryl Tryptose Broth. Incorporating MUG into Violet Red Bile Agar permits the detection of E. coli among the coliform colonies. Standard methods procedures specify Violet Red Bile Agar with MUG for detecting E. coli in food and dairy products by fluorescence. This medium is often abbreviated as VRBA w/ MUG.
|Enzymatic Digest of Gelatin||7 g|
|Yeast Extract||3 g|
|Bile Salts Mixture||1.5 g|
|Sodium Chloride||5 g|
|Neutral Red||0.03 g|
|Crystal Violet||0.002 g|
Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
- Suspend 41.6 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for only two minutes to completely dissolve the medium.
- Cool the medium to 45 – 46°C and pour plates.
|Product #||Product Description|
|7359A||Violet Red Bile Agar with MUG, 500 g|
|7359T||Violet Red Bile Agar with MUG, 5 kg|
7359 - Violet Red Bile Agar with MUG, Product Information Sheet [English] [Portuguese]
7359 - Violet Red Bile Agar with MUG, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
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