Product number: 7347

Tryptose Agar is used for the cultivation of Brucella spp. and other fastidious microorganisms in a laboratory setting. Tryptose Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Tryptose Agar is recommended for the cultivation and isolation of pathogenic and saprophytic bacteria, especially Brucella as specified by Huddleson and Castaneda. Huddleson found Tryptose media to be equal or superior to meat infusion media, providing uniformity for the cultivation and differentiation of fastidious organisms. The successful isolation of Brucella spp. supports the use of Tryptose media as a rich general purpose media. Tryptose Agar is prepared with Tryptose, which is a mixed enzymatic hydrolysate with rich nutritional value for microbiological culture media. The enzymatic hydrolysis of Tryptose permits the preservation of vitamins and amino acids.

Tryptose Agar is specified in the Compendium of Methods for the Microbiological Examination of Foods. Tryptose media are recommended in the FDA Bacteriological Analytical Manual(BAM) for serological testing.


Formula Liter
Tryptose 20 g
Dextrose 1 g
Sodium Chloride 5 g
Agar 15 g

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 41 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7347A Tryptose Agar, 500 g

Reference Material

7347 - Tryptose Agar, Product Information Sheet [English] [Portuguese]

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