Product number: 7654

Tryptic Soy Blood Agar Base No. 2 is used with blood for the isolation and cultivation of a wide variety of microorganisms in a laboratory setting. Tryptic Soy Blood Agar Base No. 2 is not intended for use in the diagnosis of disease or other conditions in humans.

Blood Agar Bases are typically supplemented with 5 – 10% sheep, rabbit, or horse blood, and used for isolating, cultivating, and determining hemolytic reactions of fastidious pathogenic microorganisms. Without enrichment, this blood agar base can be used as a general purpose medium.

Tryptic Soy Agar is based on the Soybean-Casein Digest formula specified by US Pharmacopeia. Tryptic Soy Agar is a nutritious base, and a variety of supplements, including blood, are often added to enhance this medium. Tryptic Soy Blood Agar Base No. 2 was formulated to achieve good growth and improve hemolytic reactions of important pathogenic bacteria. Blood Agar Base media are specified in standard methods for food testing.


Formula Liter
Enzymatic Digest of Casein, Type H 15 g
Enzymatic Digest of Soybean Meal 5 g
Sodium Chloride 5 g
Agar 15 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 40 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Prepare 5 – 10% blood agar by aseptically adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium, cooled to 45 – 50°C.

Order Details

Product # Product Description
7654A Tryptic Soy Blood Agar Base No. 2, 500 g
7654T Tryptic Soy Blood Agar Base No. 2, 5 kg

Reference Material

NCM0098 - Tryptic Soy Blood Agar Base No. 2, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish] [Swedish]
7654 - Tryptic Soy Blood Agar Base No. 2, Product Information Sheet [English]

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