Product number: 7154

Schaedler Broth is used for the cultivation of anaerobic microorganisms in a laboratory setting. Schaedler Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Survival of anaerobic bacteria is dependent on their sensitivity to oxygen, nutritional requirements, appropriate collection, culture medium, and incubation time and temperature. Schaedler Broth is suitable for standard procedures used in cultivating anaerobic bacteria.

Schaedler Broth is prepared according to the formulation described by Schaedler, Dubos, and Costello, and modified by Mata, Carrillo, and Villatoro. Modifications include reduced dextrose to avoid interference with hemolytic reactions, reduced yeast extract to avoid darkening of the medium, and adjusted sodium chloride and nitrogen concentrations.

Stalons, Thornsberry, and Dowell evaluated nine broth media in varied carbon dioxide atmospheres for their ability to support growth of anaerobic bacteria. Schaedler Broth in an atmosphere of 5% CO2, 10% hydrogen, and 85% nitrogen exhibited the fastest and highest growth response.

 

Formula Liter
Enzymatic Digest of Casein 5.6 g
Enzymatic Digest of Soybean Meal 1 g
Enzymatic Digest of Animal Tissue 5 g
Yeast Extract 5 g
Sodium Chloride 1.7 g
Potassium Phosphate 0.82 g
Dextrose 5.82 g
Tris (hydroxymethyl) Aminomethane 3 g
Hemin 0.01 g
L-Cystine 0.4 g

Final pH: 7.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Dissolve 28.4 g of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7154A Schaedler Broth, 500 g
7154C Schaedler Broth, 10 kg

Reference Material

7154 - Schaedler Broth, Product Information Sheet [English]
7154 - Schaedler Broth, Safety Data Sheet [English]

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