SCCRAM Broth is a non-selective culture medium used for the rapid enrichment of Salmonella spp. with the ISO-GRID® and/or NEO-GRID® Membrane Filtration System in a laboratory setting. SCCRAM Broth is not intended for use in the diagnosis of disease or other conditions in humans.
SCCRAM Broth was developed for the primary non-selective enrichment of Salmonella spp. from food samples. This medium was designed to stimulate sufficient repair and multiplication of Salmonella spp. within 6 – 8 hours of incubation at 42°C. The SCCRAM Broth formulation is used to enable direct presumptive detection of Salmonella in a total incubation period of 24 hours using EF-18 Agar and the ISO-GRID and/or NEO-GRID Membrane Filtration System.
|Special Peptone||10 g|
|Sodium Pyruvate||5 g|
|Sodium Chloride||5 g|
|MOPS, free acid||10.5 g|
|MOPS, sodium salt||11.6 g|
Final pH: 7.1 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Supplement, if desired
Tween 80, 100 mL
- Suspend 42.1 g of the medium in one liter of purified water.
- If desired, add 10 g of Tween 80.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes.
|Product #||Product Description|
|6910A||SCCRAM Broth, 500 g|
|7992||Tween 80, 100 mL|