Product number: 7392

Sabouraud Dextrose Agar with Lecithin & Tween 80 is used for the isolation of fungi from surfaces sanitized with quaternary ammonium compounds in a laboratory setting. Sabouraud Dextrose Agar with Lecithin & Tween 80 is not intended for use in the diagnosis of disease or other conditions in humans.

Sabouraud Dextrose Agar (SDA) is a modification of Dextrose Agar described by Sabouraud. SDA is used for cultivating pathogenic and commensal fungi and yeasts. The high dextrose concentration and acidic pH of the formula permits selectivity of fungi. The addition of Lecithin and Tween 80 to Sabouraud Dextrose Agar is used to neutralize antiseptics and disinfectants for environmental monitoring and other applications. Complete neutralization of disinfectants is important. Disinfectant carryover can cause false no-growth test results.

 

Formula Liter
Enzymatic Digest of Casein 5 g
Enzymatic Digest of Animal Tissue 5 g
Dextrose 40 g
Lecithin 0.7 g
Tween 80 5 g
Agar 15 g

Final pH: 5.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 71 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes. DO NOT OVERHEAT.
  4. After cooling to 45 – 50°C aseptically pour approximately 17 mL into 65 x 15 mm plates to give a meniscus of agar which extends above the top of the plate.

Order Details

Product # Product Description
7392A Sabouraud Dextrose Agar with Lecithin & Tween 80, 500 g
7392T Sabouraud Dextrose Agar with Lecithin & Tween 80, 5 kg
7392C Sabouraud Dextrose Agar with Lecithin & Tween 80, 10 kg

Reference Material

7392 - Sabouraud Dextrose Agar with Lecithin & Tween 80, Product Information Sheet [English] [Portuguese]
7392 - Sabouraud Dextrose Agar with Lecithin & Tween 80, Safety Data Sheet [English] [Portuguese]

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