Product number: 7458

Pseudomonas cepacia Agar is used for the selective isolation and detection of Pseudomonas cepacia (Burkholderia cepacia) from samples in a laboratory setting. Pseudomonas cepacia Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Pseudomonas cepacia was first described as a phytopathogen, and isolated from rotting onions and various soil and animal sources In recent years Pseudomonas cepacia has become an opportunist, causing nosocomial infections including endocarditis, pneumonia, wound, and urinary tract infections. This organism has been isolated from disinfectants, nebulizers, incubators, and intraveous fluids in hospitals worldwide. P. cepacia causes chronic lung infections in cystic fibrosis patients, and can lead to life-threatening systemic complications. P. cepacia is highly resistant to aminoglycosides and other antibiotics, making it difficult to treat.

In 1985 Gilligan et al. developed Pseudomonas cepacia Agar. This medium has shown good recovery of P. cepacia, even among mixed populations. Some P. cepacia strains produce a nonfluorescent phenazine pigment, while others nonpigmented. A sweet odor similar to that associated with P. aeruginosa is produced by some strains.

 

Formula Liter
Enzymatic Digest of Animal Tissue 1 g
Sodium Pyruvate 5 g
Bile Extract 1.5 g
Ammonium Sulfate 1 g
Ferrous Ammonium Sulfate 0.01 g
Magnesium Sulfate 0.2 g
Dipotassium Phosphate 4.3 g
Monopotassium Phosphate 2.1 g
Phenol Red 0.02 g
Crystal Violet 0.001 g
Agar 15 g

Final pH: 7.1 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplement (10 mL) / Liter

Ticarcillin (100 mg)
Polymyxin B (300,000 units)

Directions

  1. Suspend 30 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 – 50°C and aseptically add a filter sterilized solution containing Ticarcillin (100 mg) and Polymyxin B (300,000 units) dissolved in 10 mL of sterile water.

Order Details

Product # Product Description
7458A Pseudomonas cepacia Agar, 500 g

Reference Material

7458 - Pseudomonas cepacia Agar, Product Information Sheet [English] [Portuguese]

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