Product number: 7286

Nutrient Agar 1.5% is used for the cultivation of a wide variety of microorganisms in a laboratory setting. Nutrient Broth 1.5% is not intended for use in the diagnosis of disease or other conditions in humans.

In the early 1900's, the American Public Health Association (APHA) suggested the formula of Nutrient Agar as a standard culture medium used in water testing. Nutrient Agar is specified in many standard method procedures. Nutrient Agar 1.5% is a modification of Nutrient Agar. Nutrient Agar 1.5% is a general purpose medium, with a slightly alkaline pH. This medium contains 0.8% sodium chloride and can be used as a base for enrichment with blood, ascitic fluid, or other supplements for cultivating fastidious microorganisms.

 

Formula Liter
Beef Extract 3 g
Enzymatic Digest of Gelatin 5 g
Sodium Chloride 8 g
Agar 15 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 31 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7286A Nutrient Agar 1.5%, 500 g

Reference Material

7286 - Nutrient Agar 1.5%, Product Information Sheet [English]
7286 - Nutrient Agar 1.5%, Safety Data Sheet [English]

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