Product number: NCM0118

W-L Nutrient Agar is used for the cultivation of yeasts, molds, and bacteria encountered in brewing and industrial fermentations. W-L Nutrient Agar is not intended for use in the diagnosis of disease or other conditions in humans.

W-L Nutrient Medium was developed by Green and Gray while studying various fermentation processes. An exhaustive study examining methods of fermentation control procedures in worts, beers, liquid yeasts and similar fermentation products led to the development of W-L Nutrient Medium. At a pH of 5.5, counts of viable baker’s yeast will grow on W-L Nutrient Medium. W-L Nutrient Medium is also referred to as “Wallerstein Laboratory Medium”.

 

Formula Liter
Yeast Extract 4.0 g/L
Enzymatic Digest of Casein 5.0 g/L
Dextrose 50.0 g/L
Monopotassium Phosphate 0.55 g/L
Potassium Chloride 0.425 g/L
Calcium Chloride 0.125 g/L
Magnesium Sulfate 0.125 g/L
Ferric Chloride 0.0025 g/L
Manganese Sulfate 0.0025 g/L
Bromocresol Green 0.022 g/L
Agar 20.0 g/L

Final pH: 5.5 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Suspend 80 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.

Product was previously known as Acumedia Product: 7488 WL Nutrient Agar; and LabM Product: LAB079 WL Nutrient Agar

Order Details

Product # Product Description
NCM0118A WL Nutrient Agar, 500 g
NCM0118B WL Nutrient Agar, 5 kg
NCM0118C WL Nutrient Agar, 10 kg
NCM0118D WL Nutrient Agar, 25 kg (European only size)
NCM0118E WL Nutrient Agar, 50 kg (US only size)

Reference Material

NCM0118 - W-L Nutrient Medium, Technical Specifications Sheet [US English]
7488 - W-L Nutrient Medium, Product Information Sheet [English] [Portuguese]
7488 - W-L Nutrient Medium, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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