Product number: NCM0041

Violet Red Bile Glucose Agar is used for the enumeration of Enterobacteriaceae in foods and is not intended for use in the diagnosis of disease or other conditions in humans.

Formulated according to ISO 21528:2017 (all parts), VRBGA is a modification of Violet Red Bile Lactose Agar (NCM0089) introduced by Mossel in 1978. VRBA contains lactose which is fermented by members of the coli/ aerogenes group, this medium gives a ‘coliform’ count. The VRBGA formula modification replaces lactose with glucose. Glucose is fermented by all members of the Enterobacteriaceae thus gives a presumptive Enterobacteriaceae count. Bile salts and crystal violet are used to inhibit Gram positive and non-enteric organisms. The overlay procedure ensures anaerobic conditions and suppresses the growth of non-fermentative Gram negative bacteria.


Formula Liter
Yeast Extract 3.0 g/L
Enzymatic digest of Animal Tissue 7.0 g/L
Bile Salts 1.5 g/L
Sodium Chloride 5.0 g/L
Glucose 10.0 g/L
Agar 12.0 g/L
Neutral Red 0.03 g/L
Crystal Violet 0.002 g/L

pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 38.5 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  4. Cool to 45-50°C.

Product was previously known as Acumedia Product: 7425 Violet Red Bile Glucose Agar; and LabM Product: LAB088 Violet Red Bile Glucose Agar (VRBGA)

Order Details

Product # Product Description
NCM0041A Violet Red Bile Glucose Agar (VRBGA), 500 g
NCM0041B Violet Red Bile Glucose Agar (VRBGA), 5 kg
NCM0041C Violet Red Bile Glucose Agar (VRBGA), 10 kg
NCM0041D Violet Red Bile Glucose Agar (VRBGA), 25 kg (European only size)
NCM0041E Violet Red Bile Glucose Agar (VRBGA), 50 kg (US only size)

Reference Material

NCM0041 - Violet Red Bile Glucose Agar (ISO), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM0041 - Violet Red Bile Glucose Agar (ISO), Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at

Please check with your local representative for availability of these products within your region.