A medium for the enumeration of coliform organisms in food and dairy products according to ISO 4832:2006 and is not intended for use in the diagnosis of disease or other conditions in humans.
The selectivity of the medium is due to the presence of bile salts and crystal violet. Lactose fermenters produce red/purple colonies often surrounded by a halo of the same color. Non-lactose fermenters produce pale colonies. Selectivity can be increased by incubation at 42-44°C.
| Formula | Liter |
| Yeast Extract | 3.0 g/L |
| Enzymatic Digest of Animal Tissue | 7.0 g/L |
| Bile Salts | 1.5 g/L |
| Lactose | 10.0 g/L |
| Neutral Red | 0.03 g/L |
| Crystal Violet | 0.002 g/L |
| Agar | 12.0 g/L |
Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Preparation
- Suspend 38.5g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- DO NOT AUTOCLAVE.
- Cool to 45-50°C.
Product was previously known as LabM Product: LAB031 Violet Red Bile Agar (VRBA)
Order Details
| Product # | Product Description |
| NCM0089A | Violet Red Bile Lactose Agar (VRBL) (ISO), 500 g |
| NCM0089B | Violet Red Bile Lactose Agar (VRBL) (ISO), 5 kg |
| NCM0089C | Violet Red Bile Lactose Agar (VRBL) (ISO), 10 kg |
| NCM0089D | Violet Red Bile Lactose Agar (VRBL) (ISO), 25 kg (European only size) |
| NCM0089E | Violet Red Bile Lactose Agar (VRBL) (ISO), 50 kg (US only size) |
Reference Material
NCM0089 - Violet Red Bile Lactose Agar (VRBL) (ISO), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM0089 - Violet Red Bile Lactose Agar (VRBL) (ISO), Technical Specifications Sheet [English]
For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.