Product number: NCM0012

UVM Broth is used for the selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans.

UVM Broth is a modification of the formula described by Donnelly and Baigent. This formula is used for the selective enrichment of Listeria spp. from food samples. Listeria spp. grow over a pH range of 5.0 - 9.6 and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation.

Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue, Beef Extract, and Yeast Extract provide nitrogen, vitamins, and minerals in UVM Broth. The Phosphates are the buffering agents, Sodium Chloride maintains osmotic balance. Nalidixic Acid inhibits growth of Gram-negative organisms. Acriflavin inhibits Gram-positive bacteria. Esculin is hydrolyzed by Listeria spp. The high salt tolerance of Listeria is used as a means to inhibit growth of enterococci.

 

Formula Liter
Enzymatic Digest of Casein 5.0 g/L
Enzymatic Digest of Animal Tissue 5.0 g/L
Beef Extract 5.0 g/L
Yeast Extract 5.0 g/L
Sodium Chloride 20.0 g/L
Disodium Hydrogen Phosphate 9.6 g/L
Potassium Dihydrogen Phosphate 1.35 g/L
Esculin 1.0 g/L
Acriflavin 0.012 g/L
Nalidixic Acid 0.02 g/L

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Dissolve 52 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Product was previously known as Acumedia Product: 7409 UVM Modified Listeria Enrichment Broth; and LabM Product: LAB155 UVM Broth Base

Order Details

Product # Product Description
NCM0012A UVM Broth, 500 g
NCM0012B UVM Broth, 5 kg
NCM0012C UVM Broth, 10 kg
NCM0012D UVM Broth, 25 kg (European only size)
NCM0012E UVM Broth, 50 kg (US only size)

Reference Material

7409 - UVM Broth, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0012 - UVM Broth, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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