Product number: NCM0177

Urea Broth Base is for the detection of urease production, and is not intended for use in the diagnosis of disease or other conditions in humans.

This is a liquid version of Christensen’s medium (NCM0180) introduced by Maslen in 1952. This modification allows inoculation by Pasteur pipette, and it is easier to detect contamination in a fluid rather than in a slope. Maslen also claimed that it is easier to detect positive results.

 

Formula Liter
Peptone 1.0 g/L
Glucose 1.0 g/L
Disodium Phosphate 1.2 g/L
Potassium Dihydrogen Phosphate 0.8 g/L
Sodium Chloride 5.0 g/L
Phenol Red 0.004 g/L

Final pH: 6.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Dissolve 0.9 grams of the medium in 95ml of purified water.
  2. Heat with frequent agitation to completely dissolve the medium if necessary.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C then add aseptically 5ml of X130/X135 sterile urea solution.

Product was previously known as LabM Product: LAB131 Urea Broth Base

Order Details

Product # Product Description
NCM0177A Urea Broth Base (Christensen), 500 g
NCM0177B Urea Broth Base (Christensen), 5 kg
NCM0177C Urea Broth Base (Christensen), 10 kg
NCM0177D Urea Broth Base (Christensen), 25 kg (European only size)
NCM0177E Urea Broth Base (Christensen), 50 kg (US only size)

Reference Material

NCM0177 - Urea Broth Base (Christensen), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0177 - Urea Broth Base (Christensen), Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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