Product number: NCM0177
Urea Broth Base is for the detection of urease production, and is not intended for use in the diagnosis of disease or other conditions in humans.
This is a liquid version of Christensen’s medium (NCM0180) introduced by Maslen in 1952. This modification allows inoculation by Pasteur pipette, and it is easier to detect contamination in a fluid rather than in a slope. Maslen also claimed that it is easier to detect positive results.
|Disodium Phosphate||1.2 g/L|
|Potassium Dihydrogen Phosphate||0.8 g/L|
|Sodium Chloride||5.0 g/L|
|Phenol Red||0.004 g/L|
Final pH: 6.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
- Dissolve 0.9 grams of the medium in 95ml of purified water.
- Heat with frequent agitation to completely dissolve the medium if necessary.
- Autoclave at 121°C for 15 minutes.
- Cool to 45-50°C then add aseptically 5ml of X130/X135 sterile urea solution.
Product was previously known as LabM Product: LAB131 Urea Broth Base
|Product #||Product Description|
|NCM0177A||Urea Broth Base (Christensen), 500 g|
|NCM0177B||Urea Broth Base (Christensen), 5 kg|
|NCM0177C||Urea Broth Base (Christensen), 10 kg|
|NCM0177D||Urea Broth Base (Christensen), 25 kg (European only size)|
|NCM0177E||Urea Broth Base (Christensen), 50 kg (US only size)|
NCM0177 - Urea Broth Base (Christensen), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0177 - Urea Broth Base (Christensen), Technical Specifications Sheet [English]
For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.