Product number: NCM0019

Tryptone Soy Broth (TSB) is a general-purpose broth which will support the growth of a wide range of micro-organisms and is not intended for use in the diagnosis of disease or other conditions in humans.

Tryptic Soy Broth, a general purpose medium, is commonly referred to as Casein Soya Bean or Soybean- Casein Digest Broth, and abbreviated as TSB. This medium was originally developed for use without blood in determining the effectiveness of sulfonamides against pneumococci and other organisms. Clostridia and non-sporulating anaerobes grow luxuriantly in this broth when incubated under anaerobic conditions. TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry.

Enzymatic Digest of Casein and Enzymatic Digest of Soybean Meal are nitrogen sources in TSB. Dextrose is the carbon energy source that facilitates organism growth. Sodium Chloride maintains osmotic balance; Dipotassium Phosphate is a buffering agent.

For Casein Soya Bean Digest Broth QC tested according to the USP/EP/JP methodology and requirements, please use NCM0004.


Formula Liter
Enzymatic Digest of Casein 17.0 g/L
Enzymatic Digest of Soybean 3.0 g/L
Sodium Chloride 5.0 g/L
Dipotassium Hydrogen Phosphate 2.5 g/L
Glucose Monohydrate 2.5 g/L

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 30 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Product was previously known as LabM Product: LAB004 Tryptone Soy Broth TSB

Order Details

Product # Product Description
NCM0019A Tryptone Soy Broth, 500 g
NCM0019B Tryptone Soy Broth, 5 kg
NCM0019C Tryptone Soy Broth, 10 kg
NCM0019D Tryptone Soy Broth, 25 kg (European only size)
NCM0019E Tryptone Soy Broth, 50 kg (US only size)

Reference Material

NCM0019 - Tryptone Soy Broth, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0019 - Tryptone Soy Broth, Technical Specifications Sheet [English]

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