Product number: NCM0004

Tryptic Soy Broth (Soybean-Casein Digest Broth) is used for the cultivation of a wide variety of microorganisms. Tryptic Soy Broth conforms to Harmonized USP/EP/JP requirements and is not intended for use in the diagnosis of disease or other conditions in humans.

Tryptic Soy Broth, a general purpose medium, is commonly referred to as Casein Soya Bean or Soybean-Casein Digest broth, and abbreviated as TSB. This medium was originally developed for use without blood in determining the effectiveness of sulfonamides against pneumococci and other organisms. Clostridia and non-sporulating anaerobes grow luxuriantly in this broth when incubated under anaerobic conditions. TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry. TSB was chosen by the USDA Animal and Plant Health Inspection Service for detecting viable bacteria in live vaccines.

TSB conforms to Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP). The rich nutritional base of TSB, supplemented with SPS and CO2, is an excellent broth for blood cultures. With the addition of 6.5% NaCl, TSB can be used for the selective growth of group D streptococci.

Enzymatic Digest of Casein and Enzymatic Digest of Soybean Meal are nitrogen sources in TSB. Dextrose is the carbon energy source that facilitates organism growth. Sodium Chloride maintains osmotic balance; Dipotassium Phosphate is a buffering agent.


Formula Liter
Enzymatic Digest of Casein 17.0 g/L
Enzymatic Digest of Soybean 3.0 g/L
Sodium Chloride 5.0 g/L
Dipotassium Hydrogen Phosphate 2.5 g/L
Glucose Monohydrate 2.5 g/L

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 30 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Product was previously known as Acumedia Product: 7164 Tryptic Soy Broth; and LabM Product: HP002 Casein Soya Bean Digest Broth (USP/EP/JP)

Order Details

Product # Product Description
NCM0004A Tryptic Soy Broth (Soybean-Casein Digest Broth), 500 g
NCM0004B Tryptic Soy Broth (Soybean-Casein Digest Broth), 5 kg
NCM0004C Tryptic Soy Broth (Soybean-Casein Digest Broth), 10 kg
NCM0004D Tryptic Soy Broth (Soybean-Casein Digest Broth), 25 kg (European only size)
NCM0004E Tryptic Soy Broth (Soybean-Casein Digest Broth), 50 kg (US only size)

Reference Material

NCM0004 - Tryptic Soy Broth (Soybean-Casein Digest Broth), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0004 - Tryptic Soy Broth (Soybean-Casein Digest Broth), Technical Specifications Sheet [English]

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