Product number: NCM0011

Tryptic Soy Agar with Lecithin and Tween 80 is used for the isolation of microorganisms from surfaces sanitized with quaternary ammonium compounds and is not intended for use in the diagnosis of disease or other conditions in humans.

In 1955, Leavitt et al. discovered Tryptic Soy Agar supported excellent growth of aerobic and anaerobic microorganisms. Tryptic Soy Agar is a nutritious base and a variety of supplements are added to enhance the medium, including Lecithin and Tween 80. The Lecithin and Tween 80 inactivate some preservatives that may inhibit bacterial growth, reducing “preservative carryover”. Tryptic Soy Agar with Lecithin and Tween 80 is recommended for determining the sanitation efficiency of containers, equipment, and work area (environmental monitoring).

 

Formula Liter
Enzymatic Digest of Casein 15.0 g/L
Enzymatic Digest of Soybean Meal 5.0 g/L
Sodium Chloride 5.0 g/L
Lecithin 0.7 g/L
Tween 80 5.0 g/L
Agar 20.5 g/L

Final pH: 7.3 ± 0.2 at 25ºC
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Suspend 51.2 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121ºC for 15 minutes.
  4. Cool to 45-50ºC.

Product was previously known as Acumedia Product: 7163 Tryptic Soy Agar with Lecithin & Tween 80; and LabM Product: LAB189 Microbial Content Test Agar (MCTA)

Order Details

Product # Product Description
NCM0011A Tryptic Soy Agar with Lecithin and Tween 80, 500 g
NCM0011B Tryptic Soy Agar with Lecithin and Tween 80, 5 kg
NCM0011C Tryptic Soy Agar with Lecithin and Tween 80, 10 kg
NCM0011D Tryptic Soy Agar with Lecithin and Tween 80, 25 kg (European only size)
NCM0011E Tryptic Soy Agar with Lecithin and Tween 80, 50 kg (US only size)

Reference Material

NCM0011 - Tryptic Soy Agar w Lecithin & Tween, Safety Data Sheet [Chinese] [Chinese, Taiwan] [Danish] [Dutch] [English] [English, Australia] [English, Canada] [English, New Zealand] [EU English] [Finnish] [French] [French, Canada] [German] [Hungarian] [Indonesian] [Italian] [Japanese] [Korean] [Malay] [Portuguese, Brazil] [Spanish, Colombia] [Spanish, Mexico] [Swedish]
NCM0011 - Tryptic Soy Agar w Lecithin & Tween, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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