Product number: NCM0002

Tryptic Soy Agar is used for the preparation and maintenance of test strains used in growth promotion tests, suitability of the counting methods and as negative controls as described in the Harmonized USP/EP/JP. It is also a support plating medium for various protocols described in the microbial enumeration test section of the Harmonized USP/EP/JP. Tryptic Soy Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Enzymatic digests of casein and soybean act as a source of nitrogen and amino acid and sodium chloride maintains the osmotic balance.


Formula Liter
Enzymatic Digest of Casein 15.0 g/L
Enzymatic Digest of Soybean 5.0 g/L
Sodium Chloride 5.0 g/L
Agar 15.0 g/L

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 40 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.

Product was previously known as Acumedia Product: 7100 Tryptic Soy Agar; and LabM Product: HP016 Casein Soya Bean Digest Agar (USP/EP/JP)

Order Details

Product # Product Description
NCM0002A Tryptic Soy Agar (Soybean-Casein Digest Agar), 500 g
NCM0002B Tryptic Soy Agar (Soybean-Casein Digest Agar), 5 kg
NCM0002C Tryptic Soy Agar (Soybean-Casein Digest Agar), 10 kg
NCM0002D Tryptic Soy Agar (Soybean-Casein Digest Agar), 25 kg (European only size)
NCM0002E Tryptic Soy Agar (Soybean-Casein Digest Agar), 50 kg (US only size)

Reference Material

NCM0002 - Tryptic Soy Agar (Soybean-Casein Digest Agar), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0002 - Tryptic Soy Agar (Soybean-Casein Digest Agar), Technical Specifications Sheet [English]

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