Product number: NCM0144

Triple Sugar Iron (TSI) Agar is used for the differentiation of microorganisms on the basis of dextrose, lactose, and sucrose fermentation and hydrogen sulfide production. Triple Sugar Iron (TSI) Agar is not intended for use in the diagnosis of disease or other conditions in humans.

This is a modification of the Krumwiede and Kohn medium of 1917 which differentiates some of the Enterobacteriaceae on the basis of four reactions; fermentation of lactose, glucose and sucrose and H2S production. This medium should be used in conjunction with a urease test to eliminate Proteus spp. when screening for Salmonella spp.


Formula Liter
Beef Extract 3.0 g/L
Yeast Extract 3.0 g/L
Peptone Mixture 20.0 g/L
Sodium Chloride 5.0 g/L
Lactose 10.0 g/L
Sucrose 10.0 g/L
Glucose 1.0 g/L
Ferric Citrate 0.3 g/L
Sodium Thiosulphate 0.3 g/L
Phenol Red 0.025 g/L
Agar 12.0 g/L

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 65 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Dispense into tubes.
  4. Autoclave at 121°C for 15 minutes.
  5. Allow to set as a slope ensuring that the slant is over a butt approximately 3 cm deep.

Product was previously known as Acumedia Product: 7162 Triple Sugar Iron (TSI) Agar; and LabM Product: LAB053 Triple Sugar Iron (TSI) Agar

Order Details

Product # Product Description
NCM0144A Triple Sugar Iron (TSI) Agar, 500 g
NCM0144B Triple Sugar Iron (TSI) Agar, 5 kg
NCM0144C Triple Sugar Iron (TSI) Agar, 10 kg
NCM0144D Triple Sugar Iron (TSI) Agar, 25 kg (European only size)
NCM0144E Triple Sugar Iron (TSI) Agar, 50 kg (US only size)

Reference Material

NCM0144 - Triple Sugar Iron Agar, Technical Specifications Sheet [US English]
7162 - Triple Sugar Iron Agar, Product Information Sheet [English] [Portuguese]
7162 - Triple Sugar Iron Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]

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