Product number: NCM0061

Todd Hewitt Broth is used for the cultivation of streptococci and other fastidious microorganisms. Todd Hewitt Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Todd Hewitt Broth was originally developed for the production of antigenic streptococcal hemolysin. Todd Hewitt Broth is prepared according to the formula described by Updyke and Nickle, who compared media for type specific extract production of group A streptococci. This study was performed using Todd Hewitt Broth prepared with infusion of fresh beef heart as a control. Results showed Todd Hewitt Broth was particularly satisfactory for growth of group A streptococci for serological typing. Todd Hewitt Broth is recommended as an enrichment medium for growth of streptococcal cells in the identification of groups A and B by IF staining. Todd Hewitt Broth was used as an enrichment broth for group A streptococci in a comparison study of a rapid antigen test.

 

Formula Liter
Heart Infusion (dehydrated) 3.1 g/L
Yeast Enriched Peptone 20.0 g/L
Dextrose 2.0 g/L
Sodium Chloride 2.0 g/L
Disodium Phosphate 0.4 g/L
Sodium Carbonate 2.5 g/L

Final pH: 7.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Dissolve 30 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Product was previously known as Acumedia Product: 7161 Todd Hewitt Broth; and LabM Product: LAB075 Todd Hewitt Broth

Order Details

Product # Product Description
NCM0061A Todd Hewitt Broth, 500 g
NCM0061B Todd Hewitt Broth, 5 kg
NCM0061C Todd Hewitt Broth, 10 kg
NCM0061D Todd Hewitt Broth, 25 kg (European only size)
NCM0061E Todd Hewitt Broth, 50 kg (US only size)

Reference Material

NCM0061 - Todd Hewitt Broth, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0061 - Todd Hewitt Broth, Technical Specifications Sheet [English]

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