Product number: NCM0052

Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar is used for the selective isolation of Vibrio cholerae and other enteropathogenic vibrios according to ISO 21872-1:2017 and ISO 21872-2:2007. Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar is not intended for use in the diagnosis of disease or other conditions in humans.

TCBS Agar is also called Vibrio Selective Agar, and prepared according to the formula of Kobayashi et al. This formula is a modification of the selective medium from Nakanishi. All pathogenic Vibrio spp., except Vibrio hollisae, will grow on TCBS Agar. This highly selective agar meets the nutritional requirements of Vibrio spp. Vibrio spp. are able to grow in media containing increased salt concentrations, and some species are halophilic. Infections have been associated with ingestion of contaminated water and consumption of contaminated shellfish or seafood. Vibrio spp. are natural inhabitants of seawater.

According to ISO 21872-2, pre-incubation in Alkaline Saline Peptone Water (ASPW) (NCM0175) is required.

 

Formula Liter
Yeast Extract 5.0 g/L
Enzymatic Digest of Casein 5.0 g/L
Enzymatic Digest of Animal Tissue 5.0 g/L
Sodium Citrate 10.0 g/L
Sodium Thiosulfate 10.0 g/L
Bile Salts 8.0 g/L
Sucrose 20.0 g/L
Sodium Chloride 10.0 g/L
Ferric Citrate 1.0 g/L
Bromothymol Blue 0.04 g/L
Thymol Blue 0.04 g/L
Agar *14.0 g/L

* 10 - 15g according to gel strength

Final pH: 8.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Suspend 88 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve medium.
  3. DO NOT AUTOCLAVE

Product was previously known as Acumedia Product: 7210 TCBS Agar; and LabM Product: LAB096 T.C.B.S. Cholera Medium

Order Details

Product # Product Description
NCM0052A Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar, 500 g
NCM0052B Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar, 5 kg
NCM0052C Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar, 10 kg
NCM0052D Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar, 25 kg (European only size)
NCM0052E Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar, 50 kg (US only size)

Reference Material

NCM0052 - Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0052 - Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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