Product number: NCM0265

Sugar Free Agar is used for the enumeration of psychrotrophic and mesophilic Gram-negative rods, and is not intended for use in the diagnosis of disease or other conditions in humans.

A formula described by the International Dairy Federation for the enumeration of psychrotrophic and mesophilic Gram negative rods in butter and other dairy products. The Gram negative rods are able to deaminate proteins as a carbon source, whilst some enterococci are inhibited by this formula. The medium conforms to the formulation of the International Dairy Federation (I.D.F.).


Formula Liter
Gelatin Peptone 7.5 g/L
Tryptone 7.5 g/L
Sodium Chloride 5.0 g/L
Agar 14.0 g/L

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 34 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.

Product was previously known as LabM Product: LAB087 Sugar Free Agar

Order Details

Product # Product Description
NCM0265A Sugar Free Agar, 500 g
NCM0265B Sugar Free Agar, 5 kg
NCM0265C Sugar Free Agar, 10 kg
NCM0265D Sugar Free Agar, 25 kg (European only size)
NCM0265E Sugar Free Agar, 50 kg (US only size)

Reference Material

NCM0265 - Sugar Free Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0265 - Sugar Free Agar, Technical Specifications Sheet [English]

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