Product number: NCM0167

Sorbitol MacConkey Agar is used for the isolation of pathogenic Escherichia coli in a laboratory setting. Sorbitol MacConkey Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Sorbitol MacConkey Agar is based on the formula by Rappaport and Henig. Originally developed for isolating enteropathogenic (EPEC) serotypes, O11 and O55, this medium is recommended for the isolation and differentiation of enterohemorrhagic E. coli O157:H7. Serotype O157 has been implicated in serious foodborne diseases.

MacConkey Agar W/ Sorbitol contains sorbitol instead of lactose for differentiating enteropathogenic E. coli serotypes; these strains are typically sorbitol negative. MacConkey Agar W/ Sorbitol is recommended for food testing.

Enzymatic Digest of Gelatin, Enzymatic Digest of Casein, and Enzymatic Digest of Animal Tissue are the nitrogen and vitamin sources in MacConkey Agar W/ Sorbitol. Sorbitol is the fermentable carbohydrate; typically enteropathogenic strains produce colorless colonies. Bile Salts Mixture and Crystal Violet are the selective agents, inhibiting Gram-positive cocci. Sodium Chloride maintains the osmotic environment, and Neutral Red is the pH indicator. Agar is the solidifying agent. The selectivity of the medium can be increased through the addition of Cefixime and tellurite.

 

Formula Liter
Enzymatic Digest of Gelatin 17.0 g/L
Enzymatic Digest of Casein 1.5 g/L
Enzymatic Digest of Animal Tissue 1.5 g/L
Sorbitol 10.0 g/L
Bile Salts Mixture 1.5 g/L
Sodium Chloride 5.0 g/L
Neutral Red 0.03 g/L
Crystal Violet 0.001 g/L
Agar 13.5 g/L

Final pH: 7.1 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Suspend 50 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°
  5. If required add 2 vials of Cefixime Tellurite supplement X161 and mix well before dispensing.

Product was previously known as Acumedia Product: 7320 MacConkey Agar with Sorbitol; and LabM Product: LAB161 Sorbitol MacConkey Agar

Order Details

Product # Product Description
NCM0167A Sorbitol MacConkey Agar, 500 g
NCM0167B Sorbitol MacConkey Agar, 5 kg
NCM0167C Sorbitol MacConkey Agar, 10 kg
NCM0167D Sorbitol MacConkey Agar, 25 kg (European only size)
NCM0167E Sorbitol MacConkey Agar, 50 kg (US only size)

Reference Material

NCM0167 - MacConkey Agar with Sorbitol, Technical Specifications Sheet [English]
7320 - MacConkey Agar with Sorbitol, Product Information Sheet [English] [Portuguese]
7320 - MacConkey Agar with Sorbitol, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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