Product number: NCM0197

Slanetz and Bartley Medium is used for the enumeration of enterococci from water samples as described in ISO 7899-2, and is not intended for use in the diagnosis of disease or other conditions in humans.

This medium was originally described by Slanetz and Bartley for the enumeration of enterococci from water samples using a membrane filtration technique, but it may also be used as a spread plate for the examination of other sample types. Enterococci reduce tetrazolium chloride to the insoluble red dye formazan, producing colonies which are dark red or maroon on the surface of the membrane or agar. This reaction is not exclusive to enterococci, and the count at this stage should be considered presumptive. Colonies may be confirmed as enterococci by demonstrating aesculin hydrolysis using Kanamycin Aesculin Azide Agar (NCM0198).

 

Formula Liter
Tryptone 20.0 g/L
Yeast Extract 5.0 g/L
Glucose 2.0 g/L
Dipotassium Hydrogen Phosphate 4.0 g/L
Sodium Azide 0.4 g/L
2,3,5 Tetrazolium Chloride 0.1 g/L
Agar 12.0 g/L

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Suspend 43.5 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.

Product was previously known as LabM Product: LAB166 Slanetz & Bartley Medium (Membrane Enterococcus Agar)

Order Details

Product # Product Description
NCM0197A Slanetz and Bartley Medium, 500 g
NCM0197B Slanetz and Bartley Medium, 5 kg
NCM0197C Slanetz and Bartley Medium, 10 kg
NCM0197D Slanetz and Bartley Medium, 25 kg (European only size)
NCM0197E Slanetz and Bartley Medium, 50 kg (US only size)

Reference Material

LAB166 - Slanetz & Bartley Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0197 - Slanetz & Bartley Agar, Technical Specifications Sheet [English]

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