Product number: NCM0172

Selenite Broth is used for the selective enrichment of Salmonella spp. in a laboratory setting. Selenite Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Selenite Broth was originated by Leifson, while observing good recovery of Salmonella spp. and reduced growth of fecal coliforms. Salmonella organisms are also injured in food-processing procedures, including exposure to low temperatures, sub-marginal heat, drying, radiation, preservatives or sanitizers.

Selenite Broth conforms with the American Public Health Association (APHA). Many modifications of Selenite Broth exist, including Selenite Cystine Broth, from the original formula described as Selenite F Broth by Leifson.


Formula Liter
Enzymatic Digest of Casein 2.5 g/L
Enzymatic Digest of Animal Tissue 2.5 g/L
Lactose 4.0 g/L
Sodium Phosphate 10.0 g/L
Sodium Selenite 4.0 g/L

Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 23 g of the medium in one liter of purified water.
  2. Heat to boiling. Avoid overheating.

Product was previously known as Acumedia Product: 7155 Selenite Broth; and LabM Product: LAB044A/LAB044B Selenite Broth Base

Order Details

Product # Product Description
NCM0172A Selenite Broth, 500 g
NCM0172B Selenite Broth, 5 kg
NCM0172C Selenite Broth, 10 kg
NCM0172D Selenite Broth, 25 kg (European only size)
NCM0172E Selenite Broth, 50 kg (US only size)

Reference Material

NCM0172 - Selenite Broth, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM0172 - Selenite Broth, Technical Specifications Sheet [English]
7155 - Selenite Broth, Product Information Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at

Please check with your local representative for availability of these products within your region.