Product number: NCM0136

Rappaport-Vassiliadis Medium with Soya is used as a selective enrichment medium for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. RVS is not intended for use in the diagnosis of disease or other conditions in humans.

Rappaport et al. originally described the broth, which was later modified by Vassiliadis et al. and optimized by van Schothorst et al. Enzymatic digest of soya provides a source of nitrogen and amino acids and sodium chloride provides osmotic pressure. The potassium phosphate components act as a buffer. Magnesium chloride and malachite green act as selective agents against non-target microorganisms. The formulation is very hygroscopic and will produce a slight exothermic reaction when mixed with water. According to ISO 6579-1:2017 subculture is performed from Buffered Peptone Water (BPW) into RVS, followed by subculture onto Xylose-Lysine Deoxycholate Agar (XLD) and another agar. This medium conforms to the performance and formulation requirements of ISO 6579-1:2017.

 

Formula Liter
Soy Peptone 4.5 g/L
Sodium Chloride 7.2 g/L
Potassium Dihydrogen Phosphate 1.26 g/L
Dipotassium Hydrogen Phosphate 0.18 g/L
Magnesium Chloride Anhydrous 13.4 g/L
Malachite Green 0.036 g/L

Final pH: 5.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Dissolve 26.58 g of the medium in one liter of purified water.
  2. Heat with frequent agitation to dissolve if necessary.
  3. Autoclave at 115°C for 15 minutes.

Product was previously known as Acumedia Product: 7730 Rappaport-Vassiliadis Medium; and LabM Product: LAB086 Rappaport-Vassiliadis Medium (RVS)

Order Details

Product # Product Description
NCM0136A Rappaport Vassiliadis Medium with Soya (RVS) (ISO), 500 g
NCM0136B Rappaport Vassiliadis Medium with Soya (RVS) (ISO), 5 kg
NCM0136C Rappaport Vassiliadis Medium with Soya (RVS) (ISO), 10 kg
NCM0136D Rappaport Vassiliadis Medium with Soya (RVS) (ISO), 25 kg (European only size)
NCM0136E Rappaport Vassiliadis Medium with Soya (RVS) (ISO), 50 kg (US only size)

Reference Material

NCM0136 - Rappaport-Vassiliadis Medium with Soya (RVS) (ISO), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0136 - Rappaport-Vassiliadis Medium with Soya (RVS) (ISO), Technical Specifications Sheet [English]

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