Product number: NCM0103

Rappaport-Vassiliadis Salmonella Enrichment Broth is used for the enrichment and selective isolation of Salmonella spp. Conforms to Harmonized USP/EP/JP Requirements. Rappaport-Vassiliadis Salmonella Enrichment Broth is not intended for use in the diagnosis of disease or other conditions in humans.

A medium recommended by the Harmonized Pharmacopoeia for the enrichment and selective isolation of Salmonella spp.from non-sterile samples. Malachite green and magnesium chloride act as selective agents, combined with high osmotic pressure and low pH to effectively inhibit non-target competitive organisms. Soya peptone provides a source of nitrogen and potassium phosphate acts as a buffer. Some Salmonella (S. typhi and S. enteritidis) are known to be sensitive to malachite green and as such may fail to grow. If these organisms are suspected an alternative selective enrichment broth (e.g. NCM0126 Mueller-Kauffmann Tetrathionate novobiocin Broth - MKTTn) should be used in parallel. This formulation is hygroscopic and will produce a slight exothermic reaction when mixed with water. According to the Harmonized Pharmacopoeia, Rappaport Vassiliadis Salmonella Enrichment Broth is used as a selective enrichment broth, with subculture performed onto Xylose Lysine Deoxycholate (XLD) agar (NCM0027).

 

Formula Liter
Soy Peptone 4.5 g/L
Sodium Chloride 8.0 g/L
Potassium Phosphate, Monobasic 0.6 g/L
Dipotassium Phosphate 0.4 g/L
Magnesium Chloride, Anhydrous* 13.58 g/L
Malachite Green 0.036 g/L

Final pH: 5.2 ± 0.2 at 25°C
* Equivalent to 29.0 g/L Magnesium Chloride hexahydrate
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Dissolve 27.2 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Dispense 10 mL into glass tubes, cap and autoclave at 115°C for 15 minutes.

Product was previously known as Acumedia Product: 7730 Rappaport-Vassiliadis Salmonella Enrichment Broth; and LabM Product: LAB086 Rappaport-Vassiliadis Medium (RVS)

Order Details

Product # Product Description
NCM0103A Rappaport Vassiliadis Salmonella Enrichment Broth, 500 g
NCM0103B Rappaport Vassiliadis Salmonella Enrichment Broth, 5 kg
NCM0103C Rappaport Vassiliadis Salmonella Enrichment Broth, 10 kg
NCM0103D Rappaport Vassiliadis Salmonella Enrichment Broth, 25 kg (European only size)
NCM0103E Rappaport Vassiliadis Salmonella Enrichment Broth, 50 kg (US only size)

Reference Material

NCM0103 - Rappaport Vassiliadis Salmonella Enrichment Broth, Safety Data Sheet [English] [EU English]
NCM0103 - Rappaport Vassiliadis Salmonella Enrichment Broth, Technical Specifications Sheet [English]

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