Product number: NCM0113

PPLO Broth Base without Crystal Violet is used with enrichments for the isolation and cultivation of Mycoplasma spp. in a laboratory setting. PPLO Broth without Crystal Violet is not intended for the use in the diagnosis of disease or other conditions in humans.

PPLO Broth Base without Crystal Violet is prepared according to the formula described by Morton and Lecce. Crystal Violet may inhibit the growth of Mycoplasma, and therefore was removed from an earlier formula described by Morton, Smith, Williams, and Eikenberg. Mycoplasma was discovered in a case of pleuropneumonia in a cow, and referred to as “pleuropneumonia-like organism” or PPLO. Mycoplasmas belong to the class of Mollicutes “soft skin”, which are the smallest free-living organisms. They are pleomorphic, varying in size from 0.2 to 0.3 μm.

 

Formula Liter
Heart Infusion 6.0 g/L
Yeast Enriched Peptone 10.0 g/L
Sodium Chloride 5.0 g/L

Final pH: 7.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Dissolve 21 g of the medium in 700 mL of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 - 50°C and aseptically add 300 mL of the appropriate sterile serum supplement.
  5. Mix thoroughly.

Product was previously known as Acumedia Product: 0113 PPLO Broth Base without Crystal Violet; and LabM Product: LAB229 PPLO Broth Base without Crystal Violet

Order Details

Product # Product Description
NCM0113A PPLO Broth Base without Crystal Violet, 500 g
NCM0113B PPLO Broth Base without Crystal Violet, 5 kg
NCM0113C PPLO Broth Base without Crystal Violet, 10 kg
NCM0113D PPLO Broth Base without Crystal Violet, 25 kg (European only size)
NCM0113E PPLO Broth Base without Crystal Violet, 50 kg (US only size)

Reference Material

NCM0113 - PPLO Broth without CV, Technical Specifications Sheet [US English]
7485 - PPLO Broth without CV, Product Information Sheet [English] [Portuguese]
7485 - PPLO Broth without CV, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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