Product number: NCM0111

Palcam Agar Base is used with supplements as a selective and differential medium for the detection and isolation of Listeria monocytogenes from food and environmental samples. Palcam Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Palcam Agar was developed by Van Netten et al in 1989 as an improved selective differential medium for the isolation of Listeria monocytogenes from food and environmental specimens. Improved selectivity is achieved by the combination of antibiotic supplements and microaerobic incubation. The double indicator system of esculin hydrolysis and mannitol fermentation aids differentiation of Listeria spp from enterococci and staphylococci which can be confused with Listeria spp on other types of culture media. Palcam Agar is listed within Annex E of ISO 11290-1:2017 as a suitable second selective agar.

 

Formula Liter
Columbia Peptone Mix 23.0 g/L
Sodium Chloride 5.0 g/L
Corn Starch 1.0 g/L
Yeast Extract 3.0 g/L
Glucose 0.5 g/L
Mannitol 10.0 g/L
Esculin 0.8 g/L
Lithium Chloride 15.0 g/L
Ferric Ammonium citrate 0.5 g/L
Phenol Red 0.08 g/L
Agar 12.0 g/L

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Suspend 71 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C
  5. Aseptically add 2 vials of P.A.C. Supplement (X144) or Palcam Supplement (7987). Mix well.

Product was previously known as Acumedia Product: 7669 PALCAM Agar; and LabM Product: LAB148 PALCAM Agar Base

Order Details

Product # Product Description
NCM0111A Palcam Agar Base, 500 g
NCM0111B Palcam Agar Base, 5 kg
NCM0111C Palcam Agar Base, 10 kg
NCM0111D Palcam Agar Base, 25 kg (European only size)
NCM0111E Palcam Agar Base, 50 kg (US only size)

Reference Material

7669 - Palcam Agar Base, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0111 - Palcam Agar Base, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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