Product number: NCM0056

Oxford Listeria Agar Base is used with antimicrobics for the selective isolation of Listeria spp. in a laboratory setting. Oxford Listeria Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in food industries. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission included turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage and river water.

Oxford Listeria Agar is prepared according to the formulation of Curtis et al. Listeria spp. grow over a pH range of 5.0 - 9.6 and survive in food products with pH levels outside these parameters.

 

Formula Liter
Columbia Blood Agar Base 39.0 g/L
Esculin 1.0 g/L
Ferric Ammonium Citrate 0.5 g/L
Lithium Chloride 15.0 g/L
Agar 2.0 g/L

Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

Oxford Listeria Agar

  1. Suspend 57.5 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 - 50°C.
  5. Aseptically add 2 vials of Oxford Listeria Supplement (7986) or Oxford Supplement (X123) and mix well before dispensing.

Modified Oxford Listeria Agar

Perform steps 1-4 above and aseptically add a filtered sterilized aqueous solution of 10 mg colistin sulfate and 20 mg moxalactam. Mix well before dispensing.

Product was previously known as LabM Products: LAB122 Listeria Isolation Medium (Oxford) & LAB206 Listeria Isolation Media

Order Details

Product # Product Description
NCM0056A Oxford Listeria Agar, 500 g
NCM0056B Oxford Listeria Agar, 5 kg
NCM0056C Oxford Listeria Agar, 10 kg
NCM0056D Oxford Listeria Agar, 25 kg (European only size)
NCM0056E Oxford Listeria Agar, 50 kg (US only size)

Reference Material

7428 - Oxford Listeria Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0056 - Oxford Listeria Agar, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

Please check with your local representative for availability of these products within your region.