Product number: NCM0054

Orange Serum Agar is used for the cultivation of aciduric microorganisms associated with spoilage of products and is not intended for use in the diagnosis of disease or other conditions in humans.

The low pH of fruit juices makes citrus fruit products susceptible to spoilage by yeasts, molds, and the bacteria Lactobacillus and Leuconostoc. In the 1950’s, Hays investigated spoilage in frozen concentrated orange juice. He found that an agar medium containing orange serum (juice) was superior to Lindegren Agar in isolating the microorganisms responsible for spoilage causing a buttermilk off-odor. Murdock, Folinazzo, and Troy found Orange Serum Agar, pH 5.4 to be a suitable medium for growing Leuconostoc, Lactobacillus, and yeasts. Orange Serum Agar is recommended for examining fruit beverages.


Formula Liter
Orange Serum 200 mL
Yeast Extract 3.0 g/L
Enzymatic Digest of Casein 10 g/L
Dextrose 4.0 g/L
Potassium Phosphate 2.5 g/L
Agar 17.0 g/L

Final pH: 5.5 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 45.5 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Product was previously known as Acumedia Product: 7587 Orange Serum Agar; and LabM Product: LAB147 Orange Serum Agar

Order Details

Product # Product Description
NCM0054A Orange Serum Agar, 500 g
NCM0054B Orange Serum Agar, 5 kg
NCM0054C Orange Serum Agar, 10 kg
NCM0054D Orange Serum Agar, 25 kg (European only size)
NCM0054E Orange Serum Agar, 50 kg (US only size)

Reference Material

NCM0054 - Orange Serum Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0054 - Orange Serum Agar, Technical Specifications Sheet [English]

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