Product number: NCM0189

Nutrient Both No. 2 is used for the cultivation of fastidious bacteria, and forms the base for Preston Broth according to ISO 10272-1:2017 for the cultivation of Campylobacter spp. This media is not intended for use in the diagnosis of disease or other conditions in humans.

This broth can also be used as the suspending medium for cooked meat granules for the cultivation of anaerobic organisms.


Formula Liter
Beef Extract 10.0 g/L
Peptone 10.0 g/L
Sodium Chloride 5.0 g/L

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 25 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium if necessary.
  3. Autoclave at 121°C for 15 minutes.
  4. When using Nutrient Broth No. 2 to prepare Preston Broth add 2 vials of X114 and 2 vials of X115. Mix well.

Product was previously known as LabM Product: LAB014 Nutrient Broth No. 2

Order Details

Product # Product Description
NCM0189A Nutrient Broth No. 2, 500 g
NCM0189B Nutrient Broth No. 2, 5 kg
NCM0189C Nutrient Broth No. 2, 10 kg
NCM0189D Nutrient Broth No. 2, 25 kg (European only size)
NCM0189E Nutrient Broth No. 2, 50 kg (US only size)

Reference Material

NCM0189 - Nutrient Broth No. 2, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0189 - Nutrient Broth No. 2, Technical Specifications Sheet [English]

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