Product number: NCM2016

Mueller Hinton Agar I is used for antimicrobial sensitivity testing by the disc diffusion method.

A medium for antimicrobial sensitivity testing by the disc diffusion method. This medium, used in the technique of Bauer and Kirby, has been adopted by the National Committee for Clinical Laboratory Standards (NCCLS) in the USA as the definitive method for susceptibility testing. The medium has a very low thymine and thymidine content, making it suitable for trimethoprim and sulphonamide testing, controlled to ensure correct zone sizes with aminoglycoside and tetracyline antibiotics. The medium was originally formulated as a heat labile protein free medium for the isolation of pathogenic Neisseriaceae.

 

Formula Liter
Acid Hydrolysed Casein 17.5 g/L
Starch 1.5 g/L
Agar 17.0 g/L

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Suspend 38 grams of powder in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.

Product is only available in certain countries. Please contact your account manager if you require furthur details.

Order Details

Product # Product Description
NCM2016A Mueller Hinton Agar I CE, 500 g
NCM2016B Mueller Hinton Agar I CE, 5 kg
NCM2016C Mueller Hinton Agar I CE, 10 kg
NCM2016D Mueller Hinton Agar I CE, 25 kg (European only size)

Reference Material

NCM2016 - Mueller Hinton Agar I CE, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM2016 - Mueller Hinton Agar I CE, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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