Product number: NCM0036

Mueller Hinton Agar I is used for antimicrobial sensitivity testing by the disc diffusion method and is not intended for use in the diagnosis of disease or other conditions in humans.

A medium for antimicrobial sensitivity testing by the disc diffusion method. This medium, used in the technique of Bauer and Kirby, has been adopted by the National Committee for Clinical Laboratory Standards (NCCLS) in the USA as the definitive method for susceptibility testing. The medium has a very low thymine and thymidine content, making it suitable for trimethoprim and sulphonamide testing, controlled to ensure correct zone sizes with aminoglycoside and tetracyline antibiotics. The medium was originally formulated as a heat labile protein free medium for the isolation of pathogenic Neisseriaceae.


Formula Liter
Acid Hydrolysed Casein 17.5 g/L
Starch 1.5 g/L
Agar 17.0 g/L

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 38 grams of powder in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.

Product was previously known as LabM Product: LAB039 Mueller Hinton Agar

Order Details

Product # Product Description
NCM0036A Mueller Hinton Agar I, 500 g
NCM0036B Mueller Hinton Agar I, 5 kg
NCM0036C Mueller Hinton Agar I, 10 kg
NCM0036D Mueller Hinton Agar I, 25 kg (European only size)
NCM0036E Mueller Hinton Agar I, 50 kg (US only size)

This product is also available with a CE mark but with restricted sales geographically. Please click here for product details.

Reference Material

LAB039CE - Mueller Hinton Agar I, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0036 - Mueller Hinton Agar I, Technical Specifications Sheet [English]

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