Product number: NCM0190
MRS Agar is a medium for the enumeration of mesophilic lactic acid bacteria according to ISO 15214:1998, and is not intended for use in the diagnosis of disease or other conditions in humans.
MRS Agar was originally developed in 1960 by de Man, Rogosa & Sharpe for the cultivation and enumeration of Lactobacillus spp. from various sources and is intended as a substitute for Tomato Juice Agar. This original formulation has been adapted and adjusted to pH 5.7 according to ISO 15214:1998.
Nutrition is provided by enzymatic digest of casein, glucose, meat & yeast extracts whilst polyoxyethylenesorbitan monooleate, magnesium and manganese sulphates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate. Used at low pH, ammonium citrate allows growth of lactobacilli whilst inhibiting a number of other organism groups.
Occasionally, sterilization of this medium at 121°C can cause the pH to fall outside of the specified pH limits 5.7 +/- 0.1. In these rare cases adjustment of the medium using acetic acid or sodium hydroxide is recommended.
|Enzymatic Digest of Casein||10.0 g/L|
|Meat Extract||10.0 g/L|
|Yeast Extract||4.0 g/L|
|Triammonium Citrate||2.0 g/L|
|Sodium Acetate||5.0 g/L|
|Magnesium Sulphate Heptahydrate||0.2 g/L|
|Manganese Sulphate Tetrahydrate||0.05 g/L|
|Dipotassium Hydrogen Phosphate||2.0 g/L|
|Polyoxyethylenesorbitan Monooleate||1.08 g/L|
Final pH: 5.7 ± 0.1 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
- Suspend 70 grams of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes.
- Cool to 45-50°C.
Product was previously known as LabM Product: LAB223 MRS Agar (ISO)
|Product #||Product Description|
|NCM0190A||MRS Agar, 500 g|
|NCM0190B||MRS Agar, 5 kg|
|NCM0190C||MRS Agar, 10 kg|
|NCM0190D||MRS Agar, 25 kg (European only size)|
|NCM0190E||MRS Agar, 50 kg (US only size)|
LAB223 - MRS Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0190 - MRS Agar, Technical Specifications Sheet [English]
For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.