Product number: NCM0196

Modified Tryptone-Soy Broth has emerged as the medium of choice for the enrichment of E. coli O157:H7 in red meats and is incorporated into ISO/TS 13136:2012. This medium is not intended for use in the diagnosis of disease or other conditions in humans.

Concern regarding E. coli O157:H7 has grown due to the severity of the disease syndromes caused, and the increase in foodborne infection, so too has the need to optimize methods for its efficient isolation. Symptoms start with severe stomach cramps and watery, bloody diarrhea, and a percentage of individuals infected will develop Haemolytic Uraemic Syndrome (HUS) leading to acute renal failure. In a comparison of four different selective broth media, MTSB was the most productive and selective for the isolation of E. coli O157:H7. mTSB is made selective for O157:H7 by including bile salts in the dehydrated medium, and the addition of novobiocin supplement (X150).


Formula Liter
Enzymatic Digest of Casein 17.0 g/L
Sodium Chloride 5.0 g/L
Dipotassium Hydrogenphosphate 4.0 g/L
D(+)-Glucose 2.5 g/L
Enzymatic Digest of Soy 3.0 g/L
Bile Salts No.3 1.5 g/L

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 33 grams of the powder in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium if necessary.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C and add 2 vials of Novobiocin supplement X150.

Product was previously known as LabM Product: LAB165 O157 Broth (MTSB)

Order Details

Product # Product Description
NCM0196A Modified Tryptone-Soy Broth, 500 g
NCM0196B Modified Tryptone-Soy Broth, 5 kg
NCM0196C Modified Tryptone-Soy Broth, 10 kg
NCM0196D Modified Tryptone-Soy Broth, 25 kg (European only size)
NCM0196E Modified Tryptone-Soy Broth, 50 kg (US only size)

Reference Material

NCM0196 - Modified Tryptone Soy Broth (mTSB), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0196 - Modified Tryptone Soy Broth (mTSB), Technical Specifications Sheet [English]

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