Product number: NCM0184

Modified Giolitti and Cantoni Broth (ISO) is used for the detection and enumeration of coagulase- positive staphylococci from food and animal feeding stuffs using the Most Probable Number (MPN) technique according to ISO 6888-3:2003. This medium is not intended for use in the diagnosis of disease or other conditions in humans.

Originally described by Giolitti and Cantoni as a medium for the enrichment of staphylococci from foodstuffs, Mossel later applied the medium to use with samples from dried milk and infant food. Optimized for use in samples where staphylococci may be stressed and/or in low numbers, growth of the target organisms is promoted by sodium pyruvate, Glycine and the high concentration of mannitol. Selectivity is achieved via lithium chloride, which inhibits Gram-negative bacilli, and potassium tellurite, which inhibits Gram-positive organisms other than staphylococci. Further selectivity is achieved by use of anaerobiosis either by pouring a plug of agar/paraffin or by incubation in a jar or incubator under anaerobic conditions. Anaerobiosis particularly inhibits the growth of Micrococcus spp.

The presence of coagulase-positive staphylococci is indicated by the reduction of tellurite, resulting in a blackening of the broth or a black precipitate. Coagulase-positive staphylococci are principally Staphylococcus aureus but may also include the species Staphylococcus intermedius and Staphylococcus hyicus.

 

Formula Liter
Peptone Mix 15.0 g/L
Yeast Extract 5.0 g/L
Lithium Chloride 5.0 g/L
Mannitol 20.0 g/L
Sodium Chloride 5.0 g/L
Glycine 1.2 g/L
Sodium Pyruvate 3.0 g/L

Final pH: 6.9 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

Single Strength:

  1. Dissolve 54.2 grams of the medium in one liter of purified water.
  2. Swirl to mix and add 1 gram of polyoxyethelene sorbitan mono-oleate (Tween 80).
  3. Heat with frequent agitation to completely dissolve.
  4. Dispense the medium in appropriate tubes and autoclave at 121°C for 15 minutes then allow to cool to 44-47°C.
  5. Prior to use add X043 1% Potassium Tellurite to give a final concentration of 0.1 g/L, e.g. add 0.1mL X043 to 9ml of single strength base. DO NOT REHEAT MEDIA CONTAINING POTASSIUM TELLURITE.

Double Strength:

  1. Dissolve 108.4 grams of the medium in one liter of purified water.
  2. Swirl to mix and add 2 grams of polyoxyethelene sorbitan mono-oleate (Tween 80).
  3. Heat with frequent agitation to completely dissolve.
  4. Dispense the medium in appropriate tubes and autoclave at 121°C for 15 minutes then allow to cool to 44-47°.
  5. Prior to use add X043 1% Potassium Tellurite to give a final concentration of 0.1g/L, e.g. add 0.2mL X043 to 9ml of double strength base. DO NOT REHEAT MEDIA CONTAINING POTASSIUM TELLURITE.

If product is to be used on day of preparation, allow to cool to 44-47ºC and use immediately after supplementation with potassium tellurite. If the medium is not used as above then the base medium must be re-heated to 100° C for 15 minutes to expel any dissolved oxygen and cooled to 44-47ºC. DO NOT REHEAT MEDIA CONTAINING POTASSIUM TELLURITE.

Product was previously known as LabM Product: LAB219 Modified Giolitti and Cantoni Broth (ISO)

Order Details

Product # Product Description
NCM0184A Modified Giolitti and Cantoni Broth (ISO), 500 g
NCM0184B Modified Giolitti and Cantoni Broth (ISO), 5 kg
NCM0184C Modified Giolitti and Cantoni Broth (ISO), 10 kg
NCM0184D Modified Giolitti and Cantoni Broth (ISO), 25 kg (European only size)
NCM0184E Modified Giolitti and Cantoni Broth (ISO), 50 kg (US only size)

Reference Material

LAB219 - Modified Giolitti & Cantoni Broth, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0184 - Modified Giolitti & Cantoni Broth, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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