Product number: NCM0126

Muller-Kauffmann Tetrathionate-Novobiocin Broth is used for the selective and differential isolation of Salmonella spp. in a laboratory setting and is included in ISO 6579:2017. This medium is not intended for use in the diagnosis of disease or other conditions in humans.

A selective enrichment medium for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. Mueller originally described a tetrathionate broth that inhibited lactose fermenting Enterobacteriaceae but not Salmonella. Kauffmann later modified the formulation to include ox bile and brilliant green to improve selectivity. Finally, Jeffries added Novobiocin to inhibit Proteus species.

Meat extract and casein provide a source of nitrogen and amino acids and sodium chloride maintain the osmotic balance. Ox bile and brilliant green act as selective agents against non-target microorganisms. Tetrathionate is generated from the sodium thiosulfate. Iodine and calcium carbonate buffer the sulfuric acid generated from tetrathionate reduction. According to ISO 6579-1:2017 subculture is performed from Buffered Peptone Water (BPW) into MKTTn, followed by subculture onto Xylose-Lysine Deoxycholate (XLD) Agar ISO and second agar. This medium conforms to the performance and formulation requirements of ISO 6579-1:2017.

 

Formula Liter
Meat Extract 4.3 g/L
Enzymatic Digest of Casein 8.6 g/L
Sodium Chloride 2.6 g/L
Calcium Carbonate 38.7 g/L
Sodium Thiosulfate (anhydrous)* 30.45 g/L
Ox Bile 4.78 g/L
Brilliant Green 0.0096 g/L

pH: 8.0 ± 0.2 (base medium)
*Equivalent to 47.8g of sodium thiosulfate pentahydrate.
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Dissolve 89.4 grams of the medium in one liter of purified water.
  2. Mix thoroughly and bring to a boil.
  3. Cool to less than 45°C before adding supplements.
  4. Supplement with 20 ml of iodine-iodide solution (see below) and 4 vials of X150 Novobiocin.
  5. Mix well and distribute into sterile containers.

Product was previously known as Acumedia Product: 9221 Muller-Kauffmann Tetrathionate-novobiocin (MKTTn) Broth; and LabM Product: LAB202 Mueller Kauffmann Tetrathionate Novobiocin Broth (MKTTn)

Order Details

Product # Product Description
NCM0126A Muller-Kaufmann Tetrathionate-Novobiocin (MKTTn) Broth, 500 g
NCM0126B Muller-Kaufmann Tetrathionate-Novobiocin (MKTTn) Broth, 5 kg
NCM0126C Muller-Kaufmann Tetrathionate-Novobiocin (MKTTn) Broth, 10 kg
NCM0126D Muller-Kaufmann Tetrathionate-Novobiocin (MKTTn) Broth, 25 kg (European only size)
NCM0126E Muller-Kaufmann Tetrathionate-Novobiocin (MKTTn) Broth, 50 kg (US only size)

Reference Material

NCM0126 - Muller-Kauffmann Tetrathionate-novobiocin Broth, Safety Data Sheet [English] [EU English]
NCM0126 - Muller-Kauffmann Tetrathionate-novobiocin Broth, Technical Specifications Sheet [English]

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