Product number: NCM0186

Minerals Modified Glutamate Broth is for the enumeration of coliforms in water and is not intended for use in the diagnosis of disease or other conditions in humans.

This medium was developed for use with the Most Probable Numbers Technique (M.P.N.) for the enumeration of coliforms in water supplies. The medium is an improved version of the chemically defined glutamic acid medium described by Gray in 1964. The product is supplied in three parts because it has been shown that separating the sodium glutamate from the base improves its stability.


Formula Liter
Lactose 20.0 g/L
Sodium Formate 0.5 g/L
L-Cystine 0.04 g/L
L(-) Aspartic Acid 0.048 g/L
L(+) Arginine 0.04 g/L
Thiamine 0.002 g/L
Nicotinic Acid 0.002 g/L
Pantothenic Acid 0.002 g/L
Magnesium Sulphate 0.2 g/L
Ferric Ammonium Citrate 0.02 g/L
Calcium Chloride 0.02 g/L
Dipotassium Hydrogen Phosphate 1.8 g/L
Bromocresol Purple 0.02 g/L

Final pH: 6.7 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 22.7 grams of base medium (NCM0186) together with 12.7 grams of sodium glutamate (NCM0181) in one liter of purified water containing 5 grams of ammonium chloride (NCM0178).
  2. Heat with frequent agitation to completely dissolve the medium if necessary.
  3. Dispense 10ml and 50ml volumes into tubes with inverted Durham tube.
  4. Sterilize by autoclaving for 10 minutes at 115°C. Alternatively heat to 100°C for 30 minutes on three successive days.

Product was previously known as LabM Product: LAB080A Minerals Modified Glutamate Medium

Order Details

Product # Product Description
NCM0186K1 Minerals Modified Glutamate Broth, 370 g

Reference Material

NCM0186 - Minerals Modified Glutamate Broth, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0186 - Minerals Modified Glutamate Broth, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at

Please check with your local representative for availability of these products within your region.