Product number: NCM0179

Minerals Modified Glutamate Agar is used as a resuscitation medium for the enumeration of β-glucuronidase-positive Escherichia coli, and is not intended for use in the diagnosis of disease or other conditions in humans.

MMGA is a highly defined medium recommended in ISO 16649-1:2018, containing various amino acids, vitamins and salts formulated to promote the recovery of stressed or damaged organisms. According to ISO 16649-1 organisms are inoculated onto a cellulose membrane overlaid on MMGA for a resuscitation stage, followed by transfer of the membrane onto Tryptone Bile Glucuronide Agar (TBX) for isolation and counting. This medium conforms to the formulation requirements of ISO 16649-1:2018.

 

Formula Liter
Lactose 10.0 g/L
Sodium Formate 0.25 g/L
L-(-)-Cystine 0.02 g/L
L-(-)-Aspartic Acid 0.024 g/L
L-(+)-Arginine 0.02 g/L
Thiamine 0.001 g/L
Nicotinic Acid 0.001 g/L
Pantothenic Acid 0.001 g/L
Magnesium Sulfate Heptahydrate 0.1 g/L
Ferric Ammonium Citrate 0.01 g/L
Calcium Chloride Dihydrate 0.01 g/L
Dipotassium Hydrogen Phosphate 0.9 g/L
Agar 12.25 g/L

Final pH: 6.7 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Suspend 23.5 grams of base medium together with 6.35 grams of sodium glutamate (NCM0181) in one liter of purified water containing 2.5 grams of ammonium chloride (NCM0178).
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.

Product was previously known as LabM Product: LAB225 MMGA (ISO)

Order Details

Product # Product Description
NCM0179A Minerals Modified Glutamate Agar (MMGA) , 500 g
NCM0179B Minerals Modified Glutamate Agar (MMGA) , 5 kg
NCM0179C Minerals Modified Glutamate Agar (MMGA) , 10 kg
NCM0179D Minerals Modified Glutamate Agar (MMGA) , 25 kg (European only size)
NCM0179E Minerals Modified Glutamate Agar (MMGA) , 50 kg (US only size)

Reference Material

NCM0179 - Minerals Modified Glutamate Agar (MMGA), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0179 - Minerals Modified Glutamate Agar (MMGA), Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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