Product number: NCM0193

MacConkey Broth Purple is used for the detection and enumeration of fecal coliforms (37°C) and E. coli (44°C). This medium is not intended for use in the diagnosis of disease or other conditions in humans.

The replacement of neutral red used in the original formulation by bromocresol purple makes the color change caused by acid producing organisms easier to read.

 

Formula Liter
Peptone 20.0 g/L
Lactose 10.0 g/L
Bile Salts 5.0 g/L
Bromocresol Purple 0.01 g/L

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Dissolve 35 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium if necessary and dispense into tubes or bottles with inverted Durham tubes.
  3. Autoclave at 121°C for 15 minutes.
  4. Prepare double strength broth (70g/l) if 50ml or 10ml amounts of inoculum are to be added to equal volumes of broth. Prepare single strength broth (35g/l) if 1ml or 0.1ml amounts of inoculum are to be added to 10ml of broth.

Product was previously known as LabM Product: LAB005 MacConkey Broth (Purple)

Order Details

Product # Product Description
NCM0193A MacConkey Broth (Purple), 500 g
NCM0193B MacConkey Broth (Purple), 5 kg
NCM0193C MacConkey Broth (Purple), 10 kg
NCM0193D MacConkey Broth (Purple), 25 kg (European only size)
NCM0193E MacConkey Broth (Purple), 50 kg (US only size)

Reference Material

NCM0193 - MacConkey Broth Purple, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0193 - MacConkey Broth Purple, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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