Product number: NCM0060

MacConkey Broth is used for the detection of coliform bacteria in milk and water. Conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans.

A medium recommended by the Harmonized USP/EP/JP for the selective enrichment of Escherichia coli from non-sterile samples. Conforms to Harmonized USP/EP/JP performance specification. Gelatin peptone provides a source of nitrogen, while lactose is a fermentable carbohydrate. Ox bile acts a selective agent inhibiting most Gram-positive organisms and bromocresol purple acts as a pH indicator. A color change from purple to yellow indicates growth of a bile tolerant, lactose fermenting organism such as Escherichia coli. According to the Harmonized USP/EP/JP, MacConkey Broth is used as a selective enrichment broth, with subculture performed onto MacConkey Agar.


Formula Liter
Pancreatic Digest of Gelatin 20.0 g/L
Lactose Monohydrate 10.0 g/L
Dehydrated Ox Bile 5.0 g/L
Bromocresol Purple 0.01 g/L

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 35 grams of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Dispense into tubes containing Durham tubes.
  4. Autoclave at 121°C for 15 minutes.

Product was previously known as Acumedia Product: 7185 MacConkey Broth; and LabM Product: HP005 MacConkey Broth (USP/EP/JP)

Order Details

Product # Product Description
NCM0060A MacConkey Broth, 500 g
NCM0060B MacConkey Broth, 5 kg
NCM0060C MacConkey Broth, 10 kg
NCM0060D MacConkey Broth, 25 kg (European only size)
NCM0060E MacConkey Broth, 50 kg (US only size)

Reference Material

NCM0060 - MacConkey Broth, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0060 - MacConkey Broth, Technical Specifications Sheet [English]

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