Product number: NCM0072

MacConkey Agar without Crystal Violet and Salt is used for the isolation and differentiation of Gram-negative enteric bacilli from specimens containing swarming strains of Proteus spp, and is not intended for use in the diagnosis of disease or other conditions in humans.

MacConkey Agar is based on the bile salt-neutral red-lactose agar of MacConkey. The original MacConkey medium was used to differentiate strains of Salmonella typhosa from members of the coliform group. Formula modifications improved growth of Shigella and Salmonella strains. These modifications include the addition of 0.5% sodium chloride, decreased agar content, altered bile salts, and neutral red concentrations. The formula modifications improved differential reactions between enteric pathogens and coliforms.

MacConkey Agar without Crystal Violet and Salt is a differential medium that restricts swarming of Proteus spp., aiding in the detection and isolation of enteric microorganisms. Sodium Chloride is deleted from the medium to provide an electrolyte deficient medium preventing Proteus spp. from spreading. In addition, this medium does not contain crystal violet allowing Staphylococcus, Enterococcus, and Mycobacterium spp. to grow.

 

Formula Liter
Enzymatic Digest of Casein 10.0 g/L
Enzymatic Digest of Gelatin 10.0 g/L
Lactose 10.0 g/L
Bile Salts 5.0 g/L
Neutral Red 0.04 g/L
Agar 12.0 g/L

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Preparation

  1. Suspend 47 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Product was previously known as Acumedia Product: 7378 MacConkey Agar without Crystal Violet & Salt; and LabM Product: LAB002 MacConkey Agar (Without Salt)

Order Details

Product # Product Description
NCM0072A MacConkey Agar without Crystal Violet and Salt, 500 g
NCM0072B MacConkey Agar without Crystal Violet and Salt, 5 kg
NCM0072C MacConkey Agar without Crystal Violet and Salt, 10 kg
NCM0072D MacConkey Agar without Crystal Violet and Salt, 25 kg (European only size)
NCM0072E MacConkey Agar without Crystal Violet and Salt, 50 kg (US only size)

Reference Material

NCM0072 - MacConkey Agar without Crystal Violet and Salt, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0072 - MacConkey Agar without Crystal Violet and Salt, Technical Specifications Sheet [English]

For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.

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