Product number: NCM2018

MacConkey Agar No. 3 is used for the cultivation of Enterobacteriaceae.

A modification recommended by the W.H.O. and the American Public Health Association for the isolation of Enterobacteriaceae from waters and sewage. The medium has been made more selective than MacConkey’s original formula by the use of crystal violet as well as bile salts. Gram positive organisms will not grow on this medium.


Formula Liter
Peptone 20.0 g/L
Lactose 10.0 g/L
Bile Salt No.3 1.5 g/L
Sodium Chloride 5.0 g/L
Neutral Red 0.03 g/L
Crystal Violet 0.001 g/L
Agar 15.0 g/L

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 51.5 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.

Product is only available in certain countries. Please contact your account manager if you require furthur details.

Order Details

Product # Product Description
NCM2018A MacConkey Agar No. 3 CE, 500 g
NCM2018B MacConkey Agar No. 3 CE, 5 kg
NCM2018C MacConkey Agar No. 3 CE, 10 kg
NCM2018D MacConkey Agar No. 3 CE, 25 kg (European only size)

Reference Material

NCM2018 - MacConkey Agar No. 3 CE, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM2018 - MacConkey Agar No. 3 CE, Technical Specifications Sheet [EU English]

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