Product number: NCM0174

MacConkey Agar No. 3 is used for the cultivation of Enterobacteriaceae and is not intended for use in the diagnosis of disease or other conditions in humans.

A modification recommended by the W.H.O. and the American Public Health Association for the isolation of Enterobacteriaceae from waters and sewage. The medium has been made more selective than MacConkey’s original formula by the use of crystal violet as well as bile salts. Gram positive organisms will not grow on this medium.


Formula Liter
Peptone 20.0 g/L
Lactose 10.0 g/L
Bile Salt No.3 1.5 g/L
Sodium Chloride 5.0 g/L
Neutral Red 0.03 g/L
Crystal Violet 0.001 g/L
Agar 15.0 g/L

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 51.5 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.

Product was previously known as LabM Product: LAB045 MacConkey Agar No 3

Order Details

Product # Product Description
NCM0174A MacConkey Agar No. 3, 500 g
NCM0174B MacConkey Agar No. 3, 5 kg
NCM0174C MacConkey Agar No. 3, 10 kg
NCM0174D MacConkey Agar No. 3, 25 kg (European only size)
NCM0174E MacConkey Agar No. 3, 50 kg (US only size)

This product is also available with a CE mark but with restricted sales geographically. Please click here for product details.

Reference Material

NCM0174 - MacConkey Agar No. 3, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0174 - MacConkey Agar No. 3, Technical Specifications Sheet [English]

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