Product number: NCM0194

MacConkey Agar No.2 is a modification of MacConkey Agar which contains bile salts No. 2 for the recognition of Enterococci, and is not intended for use in the diagnosis of disease or other conditions in humans.

This agar especially useful when looking for Enterococci in the presence of coliforms and non-lactose fermenters from water, sewage and food products. Enterococci are frequently sought as an index of fecal pollution and appear on this medium as small, intensely colored red-purple colonies. Non-lactose fermenters appear colorless, whilst bile tolerant Gram-positive organisms, such as staphylococci and non-fecal streptococci, are completely inhibited.


Formula Liter
Peptone 20.0 g/L
Lactose 10.0 g/L
Bile Salts No. 2 1.5 g/L
Sodium Chloride 5.0 g/L
Neutral Red 0.05 g/L
Crystal Violet 0.001 g/L
Agar 15.0 g/L

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 51.6 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121oC for 15 minutes
  4. Cool to 45-50oC.

Product was previously known as LabM Product: LAB216 MacConkey Agar No.2

Order Details

Product # Product Description
NCM0194A MacConkey Agar No. 2, 500 g
NCM0194B MacConkey Agar No. 2, 5 kg
NCM0194C MacConkey Agar No. 2, 10 kg
NCM0194D MacConkey Agar No. 2, 25 kg (European only size)
NCM0194E MacConkey Agar No. 2, 50 kg (US only size)

This product is also available with a CE mark but with restricted sales geographically. Please click here for product details.

Reference Material

NCM0194 - MacConkey Agar No. 2, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM0194 - MacConkey Agar No. 2, Technical Specifications Sheet [English]

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